Reconstructed Organ Products

We develop organ-specific products based on our reconstruct organ technology where the microenvironment of each tissue is reconstructed ex vivo. Each reconstructed environment is available in 2 formats: a 3D Bioassay (comprehensive system with extracellular matrix, medium additives, and buffers) and a Basic Matrix Pack (extracellular matrix-only).

Reconstructed Bone Marrow (rBone)

rBone system recapitulates the comprehensive 3D microenvironment of the human bone marrow (both cellular, hematopoietic and stromal, and extracellular, soluble factors and extracellular matrix (ECM), compartments). rBone™ recapitulates the ECM composition of both the bone marrow and endosteum (connective tissue at the interface between the bone and the bone marrow). In rBone, the hematopoietic and stromal (osteoblasts, osteoclasts, adipocytes, and stromal cells) compartments undergo robust expansion while retaining the composition and 3D architecture of in vivo tissue. rBone maintains primary bone marrow cells for at least 21 days without loss of viability, and as such provides a unique platform to predict long-term effects and efficacy of new therapeutics under physiological and pathological conditions of human tissue microenvironment.

rBone™ matrix maintains architecture of in vivo bone marrow as demonstrated by cryo-scanning electron microscopy.

Workflow

rBone™ kit contents:

rBone™ matrix

extracellular matrix (ECM) proteins formulated to recapitulate the ECM composition of the human bone marrow   .

 

rBone™ supplement...

growth medium supplement representing the growth factor/hormone/cytokine microenvironment of the bone marrow

rEndosteum™ matrix

extracellular matrix (ECM) formulated to recapitulate the composition of the human endosteum (connective tissue between bone and bone marrow).

 

Cell Resuspension solution

a buffer for resuspending the cells for embedding in rBone™

rBone™ cocktail

growth medium additives formulated to supply extracellular factors to reconstruct bone marrow microenvironment

 

Cell Isolation solution

a buffer used to isolate viable cells from the rBone™

Ordering information: PD-3D™ kits

Reconstructed Bone Marrow (rBone™)

Product

 rBone™ Basic 3D Matrix Pack      

 

 

rBone™ 3D Bioassay

Catalog #

01017-1ML

01017-5ML

01017-10ML

01018-5ML

# tests (96-/48-/24-well)

30  / 10 / 5

150/ 50 / 25

300 / 100 / 50

150 / 50 / 25

rBone™ Multiple Myeloma (MM) pdf

 rBone™ Basic 3D Matrix Pack      

 

 

rBone™ 3D Bioassay

10011-1ML

10011-5ML

10011-10ML

10012-5ML

30  / 10 / 5

150/ 50 / 25

300 / 100 / 50

150 / 50 / 25

rBone™ Acute Myelogenous Leukemia (AML)pdf

 rBone™ Basic 3D Matrix Pack      

 

 

rBone™ 3D Bioassay

10013-1ML

10013-5ML

10013-10ML

10014-5ML

30  / 10 / 5

150/ 50 / 25

300 / 100 / 50

150 / 50 / 25

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