Reconstructed Organ is a set of organ-specific 3D culture platforms formulated based on the composition of the native microenvironment of human tissues. Each platform is available in 2 formats: a comprehensive 3D Bioassay and Basic 3D Matrix Pack (extracellular matrix only).
Add the extracellular matrix (ECM) mimicking the basement membrane or bone endosteum (r-Endosteum) to the plate to create a thin coating.
Mix cells with organ-specific ECM. Reconstructed Organ platform is compatible with multiple cell types from tumor and healthy tissues including epithelium, stroma, endothelium, and immune cells.
Add the cell/ECM mixture to the pate. Once polymerized it creates a 1mm semi-solid layer.
Overlay the cell/ECM layer with growth medium containing disease-specific factors.
Add therapeutic agents either alone or in combinations. The system is compatible with multiple drug classes including small molecules, antibodies, antibody-drug conjugates (ADC), cellular and immunotherapies, such as CAR-T, etc.
After culture, cellular behavior can be evaluated directly in-matrix or cells can be isolated from the ECM for downstream analysis.
Reconstructed Organ platform is compatible with all standard readouts such as in situ imaging, immunohistochemistry, flow cytometry, cell-based assays, genenomics, proteomics, metabolomics, etc. Cells maintain viability after isolation from the ECM and can be used for in vivo studies.
extracellular matrix (ECM) formulated to recapitulate the composition of the human endosteum (connective tissue between bone and bone marrow).
extracellular matrix (ECM) proteins formulated to recapitulate the ECM composition of the human bone marrow.
growth medium supplement representing the growth factor / hormone / cytokine microenvironment of the bone marrow
growth medium additives formulated to supply extracellular factors to reconstruct bone marrow microenvironment
Cell Isolation Solution
a buffer used to isolate viable cells from the r-Bone
Multiple Myeloma (MM)
r-Bone™ 3D Bioassay
Acute Myelogenous Leukemia (AML)
r-Bone™ 3D Bioassay
Reconstructed Bone Marrow
r-Bone™ Basic 3D Matrix Pack (ECM only)